目的 综述硫酸多糖类药物含量测定方法研究进展。方法 查阅近年来国内外研究文献, 对硫酸多糖类药物的含量测定方法如直接染色法、酸降解-显色法、光度滴定法、液相色谱法和生物效价法进行介绍, 比较各方法的特点。结果与结论 每种方法的专属性、精密度和准确性、实际操作可行性以及仪器花费各有差别, 但高效液相色谱法以其专属性好、灵敏度和准确度高等优势能够更真实地反映药物含量, 因此在药物含量测定中应用更为广泛, 是未来含量测定的主要方法。
Abstract
OBJECTIVE To review the progress in the content assay METHODS of sulfate polysaccharide drugs. METHODS By consulting the literature at home and abroad in recent years, the content assay METHODS of sulfate polysaccharide drugs, including direct staining method, acid-degradation color-development method, photometric titration, liquid chromatography and biopotency, are introduced and compared. RESULTSAND CONCLUSION The specificity, precision and accuracy, feasibility of practical operation and the instrument cost of the five METHODS are different. But high performance liquid chromatography method with good specificity, high sensitivity, and accuracy can give more accurate result of the drug content. It is more widely applied in the determination of drug content, so it will be the main method of content assay in the future.
关键词
硫酸多糖 /
肝素 /
硫酸软骨素 /
藻酸双酯钠 /
甘糖酯 /
含量测定
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Key words
sulfate polysaccharide /
heparin /
chondroitin sulfate /
propylene glycol alginate sodium sulfate /
propylene glycol mannuronate sulfate /
content assay
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参考文献
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脚注
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基金
山东省科技发展计划(2011GSF11815);青岛市公共领域科技支撑计划项目(11-2-3-73-jh)
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